IHC
Immunohistochemistry of paraffin-embedded human colon cancer using ATP2B2 Antibody at dilution of 1:100ICC/IF
Immunofluorescent analysis of 293 cells using ATP2B2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)| Product Name | Rabbit anti-ATP2B2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Plasma membrane calcium-transporting ATPase 2 protein (1-80AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2B2 |
|---|---|
| Gene Synonyms | PMCA2 DFNA82 PMCA2a PMCA2i |
| Gene Full Name | ATPase plasma membrane Ca2+ transporting 2 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | AT2B2_HUMAN antibody atp2b2 antibody ATPase plasma membrane Ca2+ transporting 2 antibody Plasma membrane calcium ATPase 2 antibody Plasma membrane calcium ATPase antibody Plasma membrane calcium ATPase isoform 2 antibody Plasma membrane calcium pump isoform 2 antibody Plasma membrane calcium transporting ATPase 2 antibody Plasma membrane calcium-transporting ATPase 2 antibody PMCA2 antibody PMCA2a antibody PMCA2i antibody |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse.; [Isoform WA]: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.; [Isoform WB]: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.; [Isoform XB]: Basolateral cell membrane; Multi-pass membrane protein.; [Isoform ZA]: Basolateral cell membrane; Multi-pass membrane protein.; [Isoform ZB]: Basolateral cell membrane; Multi-pass membrane protein. |
IHC
Immunohistochemistry of paraffin-embedded human colon cancer using ATP2B2 Antibody at dilution of 1:100
ICC/IF
Immunofluorescent analysis of 293 cells using ATP2B2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)| Application Notes | IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
