ICC/IF
Immunofluorescence staining of Hela cells with SLC12A3 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-SLC12A3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Solute carrier family 12 member 3 protein (791-952AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SLC12A3 |
|---|---|
| Gene Synonyms | NCC TSC NCCT |
| Gene Full Name | solute carrier family 12 member 3 |
| Gene Summary | This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLJ96318 antibody Na Cl cotransporter antibody Na Cl symporter antibody Na-Cl symporter antibody NaCl electroneutral thiazide sensitive cotransporter antibody NCC antibody NCCT antibody S12A3_HUMAN antibody slc12a3 antibody Solute carrier family 12 (sodium/chloride transporters) member 3 antibody Solute carrier family 12 member 3 antibody Thiazide sensitive Na Cl cotransporter antibody Thiazide sensitive sodium chloride cotransporter antibody Thiazide-sensitive sodium-chloride cotransporter antibody TSC antibody |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. |
ICC/IF
Immunofluorescence staining of Hela cells with SLC12A3 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
