WB
37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with (SLC12A3/NCCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | SLC12A3/NCCT Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:1000-1:4000 IHC:1:500-1:2000 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | SLC12A3 |
|---|---|
| Gene Synonyms | NCC TSC NCCT |
| Gene Full Name | solute carrier family 12 member 3 |
| Gene Summary | This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | SLC12A3 NCCT |
| Molecular Weight(MW) | 114 kDa(Observed band size:150 kDa) |
| Cellular Localization | Cell membrane,Membrane |
WB
37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with (SLC12A3/NCCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:4000 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
