WB
Western blot analysis of LIAS using anti-LIAS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LIAS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to LIAS |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human LIAS recombinant protein (Position: M1-K368) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIAS |
|---|---|
| Gene Synonyms | LS LAS LIP1 PDHLD HGCLAS HUSSY-01 |
| Gene Full Name | lipoic acid synthetase |
| Gene Summary | The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. Localized in the mitochondrion, this iron-sulfur enzyme catalyzes the final step in the de novo pathway for the biosynthesis of lipoic acid, a potent antioxidant. The deficient expression of this enzyme has been linked to conditions such as diabetes, atherosclerosis and neonatal-onset epilepsy. Alternative splicing occurs at this locus, and several transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Aug 2020] |
| Alternative Names | HUSSY 01 LAS LIAS Lip syn LIP1 Lipoate synthase Lipoic acid synthase lipoic acid synthetase Lipoyl synthase mitochondrial LS |
| Molecular Weight(MW) | 42 kDa(Observed band size: 39 kDa) |
| Cellular Localization | Mitochondrion |
WB
Western blot analysis of LIAS using anti-LIAS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LIAS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of LIAS using anti-LIAS antibody. LIAS was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-LIAS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
