WB
Western blot analysis of extracts of various cell lines, using Phospho-SERCA2/ATP2A2-Thr484 antibody at 1:1000 dilution.NIH/3T3 cells were treated by ATP at 30℃ for 1 hour.C6 cells were treated by ATP at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit . Exposure time: 1s.| Product Name | Phospho-SERCA2/ATP2A2-Thr484 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphorylated peptide around T484 of SERCA2/ATP2A2(NP_733765.1). |
| Modification | p-Thr484 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2A2 |
|---|---|
| Gene Synonyms | DD DAR ATP2B SERCA2 RHABDO2 |
| Gene Full Name | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 |
| Gene Summary | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019] |
| Molecular Weight(MW) | 114KkDa(Observed band size:140kDa) |
| Cellular Localization | Endoplasmic reticulum,Membrane,Sarcoplasmic reticulum |
WB
Western blot analysis of extracts of various cell lines, using Phospho-SERCA2/ATP2A2-Thr484 antibody at 1:1000 dilution.NIH/3T3 cells were treated by ATP at 30℃ for 1 hour.C6 cells were treated by ATP at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit . Exposure time: 1s.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
