WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SERCA2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human cardiac muscle was stained with anti-SERCA2 Rabbit antibodyIHC
Human kidney was stained with anti-SERCA2 Rabbit antibodyIHC
Mouse cardiac muscle was stained with anti-SERCA2 Rabbit antibody| Product Name | SERCA2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:200-1:1000 WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATP2A2 |
|---|---|
| Gene Synonyms | DD DAR ATP2B SERCA2 RHABDO2 |
| Gene Full Name | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 |
| Gene Summary | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019] |
| Alternative Names | ATP2A2 ATP2B Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 SERCA2 SR Ca 2+ -ATPase 2 Calcium pump 2 Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform Endoplasmic reticulum class 1/2 Ca 2+ ATPase |
| Molecular Weight(MW) | 115 kDa(Observed band size: 140 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SERCA2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human cardiac muscle was stained with anti-SERCA2 Rabbit antibody
IHC
Human kidney was stained with anti-SERCA2 Rabbit antibody
IHC
Mouse cardiac muscle was stained with anti-SERCA2 Rabbit antibody| Application Notes | IHC:1:200-1:1000 WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
