WB
Western blot analysis of SHANK3 on different lysates with Rabbit anti-SHANK3 antibody at 1/5,000 dilution.Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Negative control: skeletal muscle. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-SHANK3 antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureIF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain (hippocampus). Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain (cortex). Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Brain (Striatum). Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: Goat anti-Rabbit IgG (H&L) - HRP, 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Brain (Striatum). Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: Goat anti-Rabbit IgG (H&L) - HRP, 20 minutes at room temperature.| Product Name | SHANK3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse SHANK3 aa 701-1,250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IF-F:1:500 IHC:1:200-1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Shank3 |
|---|---|
| Gene Synonyms | Spank-2 proSAP2 |
| Gene Full Name | SH3 and multiple ankyrin repeat domains 3 |
| Gene Summary | Enables ionotropic glutamate receptor binding activity and scaffold protein binding activity. A structural constituent of postsynaptic density. Involved in several processes, including learning or memory; modulation of chemical synaptic transmission; and postsynapse organization. Acts upstream of or within several processes, including MAPK cascade; negative regulation of actin filament bundle assembly; and regulation of AMPA glutamate receptor clustering. Located in neuron spine and plasma membrane. Is active in glutamatergic synapse. Is expressed in central nervous system. Used to study Phelan-McDermid syndrome; autism spectrum disorder; and schizophrenia. Human ortholog(s) of this gene implicated in Phelan-McDermid syndrome and schizophrenia 15. Orthologous to human SHANK3 (SH3 and multiple ankyrin repeat domains 3). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SH3 and multiple ankyrin repeat domains protein 3 Shank3 Proline-rich synapse-associated protein 2 (ProSAP2) SPANK-2 Shank3 Kiaa1650 Prosap2 |
| Molecular Weight(MW) | 185 kDa(Observed band size: 180-220 kDa) |
| Cellular Localization | Cytoplasm, Synapse, Postsynaptic density, Cell projection, dendritic spine. |
WB
Western blot analysis of SHANK3 on different lysates with Rabbit anti-SHANK3 antibody at 1/5,000 dilution.Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Negative control: skeletal muscle. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-SHANK3 antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain (hippocampus). Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain (cortex). Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Brain (Striatum). Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: Goat anti-Rabbit IgG (H&L) - HRP, 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Brain (Striatum). Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Rabbit anti-SHANK3 antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: Goat anti-Rabbit IgG (H&L) - HRP, 20 minutes at room temperature.| Application Notes | WB:1:5000 IF-F:1:500 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
