WB
Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with TFIP11 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using TFIP11 antibody at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | TFIP11 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TFIP11 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC :1:250-1:1000 ICC/IF:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TFIP11 |
|---|---|
| Gene Synonyms | NTR1 STIP TIP39 STIP-1 Spp382 bK445C9.6 |
| Gene Full Name | tuftelin interacting protein 11 |
| Gene Summary | This gene encodes a protein component of the spliceosome that promotes the release of the lariat-intron during late-stage splicing through the recruitment of a pre-mRNA splicing factor called DEAH-box helicase 15. The encoded protein contains a G-patch domain, a hallmark of RNA-processing proteins, that binds DEAH-box helicase 15. This protein contains an atypical nuclear localization sequence as well as a nuclear speckle-targeting sequence, enabling it to localize to distinct speckled regions within the cell nucleus. Polymorphisms in this gene are associated with dental caries suggesting a role in amelogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016] |
| Alternative Names | 1A1C8 bK445C9.6 hNtr1 HSPC006 NTR1 |
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Cytoplasm,Nucleus,Spliceosome |
WB
Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with TFIP11 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using TFIP11 antibody at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC :1:250-1:1000 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
