WB
Various lysates were subjected to SDS PAGE followed by western blot with REDD1 specific antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.WB
WB result of REDD1 antibody (1:1000) with si-control and si-REDD1 transfected PC-3 cells.IHC
Immunohistochemical analysis of paraffin-embedded human heart using REDD1 specific antibody at dilution of 1:100 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human liver using REDD1 specific antibody at dilution of 1:50 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using REDD1 specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-REDD1 specific (IP: 3ug; Detection: 1:500) with MCF-7 cells lysate 2500ug.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | REDD1/DDIT4 specific Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human REDD1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:12000 IHC :1:50-1:500 ICC/IF:1:200-1:800 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDIT4 |
|---|---|
| Gene Synonyms | Dig2 REDD1 REDD-1 |
| Gene Full Name | DNA damage inducible transcript 4 |
| Gene Summary | Predicted to enable 14-3-3 protein binding activity. Involved in negative regulation of TOR signaling and response to hypoxia. Located in cytosol. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | REDD1 specific DDIT4 REDD1 DNA damage-inducible transcript 4 protein HIF-1 responsive protein RTP801 |
| Molecular Weight(MW) | 25 kDa (Observed band size: 32-35 kDa) |
| Cellular Localization | Cytoplasm,Mitochondrion |
WB
Various lysates were subjected to SDS PAGE followed by western blot with REDD1 specific antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB
WB result of REDD1 antibody (1:1000) with si-control and si-REDD1 transfected PC-3 cells.
IHC
Immunohistochemical analysis of paraffin-embedded human heart using REDD1 specific antibody at dilution of 1:100 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human liver using REDD1 specific antibody at dilution of 1:50 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using REDD1 specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-REDD1 specific (IP: 3ug; Detection: 1:500) with MCF-7 cells lysate 2500ug.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:2000-1:12000 IHC :1:50-1:500 ICC/IF:1:200-1:800 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
