WB
Western blot analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GANP/MCM3AP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody and anti-Beta Tubulin antibody.GANP/MCM3AP was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-GANP/MCM3AP Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of HEL cells using anti-GANP/MCM3AP antibody .Overlay histogram showing HEL cells stained with anti-GANP/MCM3AP antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GANP/MCM3AP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to GANP/MCM3AP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GANP/MCM3AP recombinant protein (Position: R1275-H1715). Human MCM3AP shares 80.9% amino acid (aa) sequence identity with mouse MCM3AP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCM3AP |
|---|---|
| Gene Synonyms | GANP SAC3 MAP80 PNRIID |
| Gene Full Name | minichromosome maintenance complex component 3 associated protein |
| Gene Summary | The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014] |
| Alternative Names | MCM3AP GANP KIAA0572 MAP80 Germinal-center associated nuclear protein GANP 80 kDa MCM3-associated protein MCM3 acetylating protein MCM3AP MCM3 acetyltransferase |
| Molecular Weight(MW) | 218 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nuclear pore complex,Nucleus |
WB
Western blot analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GANP/MCM3AP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody and anti-Beta Tubulin antibody.GANP/MCM3AP was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-GANP/MCM3AP Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of HEL cells using anti-GANP/MCM3AP antibody .Overlay histogram showing HEL cells stained with anti-GANP/MCM3AP antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GANP/MCM3AP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
