WB
Western blot analysis was performed using anti-EV71 VP3/Capsid protein VP3 polyclonal antibody at 1ug/mL on recombinant EV71 VP3/Capsid protein VP3.| Product Name | Anti-EV71 VP3/Capsid protein VP3 Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli - derived recombinant EV71 VP3/Capsid protein VP3 (Gly324-Gln565). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Genome polyprotein, P1, Capsid protein VP3, P1C, Virion protein 3 |
|---|---|
| Function | Genome polyprotein (VP3/Capsid protein VP3) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization. |
WB
Western blot analysis was performed using anti-EV71 VP3/Capsid protein VP3 polyclonal antibody at 1ug/mL on recombinant EV71 VP3/Capsid protein VP3.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Storage Buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
