WB
Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody at 1/1,000 dilution. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of NrCAM on human brain tissue lysates with Rabbit anti-NrCAM antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | NrCAM Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human NrCAM aa 821-970 / 1,304. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NRCAM |
|---|---|
| Gene Synonyms | NEDNMS |
| Gene Full Name | neuronal cell adhesion molecule |
| Gene Summary | Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | Bravo antibody hBravo antibody Neuronal cell adhesion molecule antibody Neuronal surface protein Bravo antibody Ng CAM related antibody Ng-CAM-related antibody NgCAM related cell adhesion molecule antibody NgCAM-related cell adhesion molecule antibody Nr CAM antibody Nr-CAM antibody Bravo antibody hBravo antibody Neuronal cell adhesion molecule antibody Neuronal surface protein Bravo antibody Ng CAM related antibody Ng-CAM-related antibody NgCAM related cell adhesion molecule antibody NgCAM-related cell adhesion molecule antibody Nr CAM antibody Nr-CAM antibody Nrcam antibody NRCAM_HUMAN antibody |
| Molecular Weight(MW) | 144 kDa |
| Cellular Localization | Cell membrane,Cell projection,,Secreted |
WB
Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody at 1/1,000 dilution. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of NrCAM on human brain tissue lysates with Rabbit anti-NrCAM antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
