WB
Western Blot: Positive WB detected in Recombinant protein. All lanes: Genome polyprotein antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.| Product Name | Genome polyprotein (BVDV)Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Bovine viral diarrhea virus Genome polyprotein protein (1-168aa ) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | Genome polyprotein [Cleaved into: N-terminal protease (N-pro) (EC 3.4.22.-) (Autoprotease p20) Capsid protein C E(rns) glycoprotein (gp44/48) Envelope glycoprotein E1 (gp33) Envelope glycoprotein E2 (gp55) p7 Non-structural protein 2-3 Cysteine protease NS2 (EC 3.4.22.-) (Non-structural protein 2) Serine protease NS3 (EC 3.4.21.113) (EC 3.6.1.15) (EC 3.6.4.13) (Non-structural protein 3) Non-structural protein 4A (NS4A) Non-structural protein 4B (NS4B) Non-structural protein 5A (NS5A) RNA-directed RNA polymerase (EC 2.7.7.48) (NS5B)] |
|---|---|
| Molecular Weight(MW) | 29 kDa |
| Function | Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.; Packages viral RNA to form a viral nucleocapsid and thereby protects viral RNA. Plays also a role in transcription regulation. Protects the incoming virus against IFN-induced effectors.; Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans.; E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis.; E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis.; Plays an essential role in the virus replication cycle by acting as a viroporin. Forms ion conductive pores, which alters the cell permeability allowing the transport of ions and other small molecules. Forms a leader sequence to properly orient NS2 in the membrane.; Uncleaved NS2-3 is required for production of infectious virus.; Plays a role in the regulation of viral RNA replication.; Multifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and viral genome replication. The chymotrypsin-like serine protease activity utilizes NS4A as an essential cofactor and catalyzes the cleavage of the polyprotein leading to the release of NS4A, NS4B, NS5A, and NS5B. Interacts with NS5B to enhance RNA-dependent RNA polymerase activity.; Acts as a cofactor for the NS3 protease activity.; Induces a specific membrane alteration that serves as a scaffold for the virus replication complex.; Replicates the viral (+) and (-) genome. |
| Cellular Localization | [Capsid protein C]: Virion.; [E(rns) glycoprotein]: Host membrane; Peripheral membrane protein. Virion membrane; Peripheral membrane protein.; [Envelope glycoprotein E2]: Host cell surface. Virion membrane.; [Cysteine protease NS2]: Host membrane; Multi-pass membrane protein.; [Serine protease NS3]: Host cytoplasm.; [Non-structural protein 4B]: Host cytoplasm.; [Non-structural protein 5A]: Host cytoplasm. |
WB
Western Blot: Positive WB detected in Recombinant protein. All lanes: Genome polyprotein antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.| Application Notes | WB:1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
