WB
Various lysates were subjected to SDS PAGE followed by western blot with WNT7A/B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using WNT7A/B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using WNT7A/B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-WNT7A (IP:4ug; Detection: 1:4000) with mouse kidney tissue lysate 2280 ug.ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).| Product Name | WNT7A/B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human WNT7A protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNT7A |
|---|---|
| Gene Synonyms | SANTOS Wnt-7a |
| Gene Full Name | Wnt family member 7A |
| Gene Summary | This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008] |
| Alternative Names | WNT7B WNT7A Protein Wnt 7a Protein Wnt-7a |
| Molecular Weight(MW) | 39 kDa (Observed band size: 30-39 kDa) |
| Cellular Localization | Extracellular matrix,Secreted |
WB
Various lysates were subjected to SDS PAGE followed by western blot with WNT7A/B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using WNT7A/B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using WNT7A/B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-WNT7A (IP:4ug; Detection: 1:4000) with mouse kidney tissue lysate 2280 ug.
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).| Application Notes | WB:1:500-1:2000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
