WB
Western blot analysis of UBE2A/2B using anti-UBE2A/2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBE2A/2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HeLa cells using anti-UBE2A/2B antibody. Overlay histogram showing HeLa cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.FC
Flow Cytometry analysis of NIH3T3 cells using anti-UBE2A/2B antibody. Overlay histogram showing NIH3T3 cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.FC
Flow Cytometry analysis of PC-12 cells using anti-UBE2A/2B antibody. Overlay histogram showing PC-12 cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to UBE2A/2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human UBE2A/2B recombinant protein (Position: M1-C152). Human UBE2A/2B shares 100% amino acid (aa) sequence identity with mouse UBE2A/2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2A |
|---|---|
| Gene Synonyms | UBC2 HHR6A MRXSN RAD6A MRXS30 |
| Gene Full Name | ubiquitin conjugating enzyme E2 A |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Alternative Names | HHR6A HR6A RAD6 homolog A RAD6A UBC2 UBE2A Ubiquitin carrier protein A Ubiquitin protein ligase A E2 17kDa HHR6B HR6B RAD6 homolog B RAD6B UBC2 UBE2B Ubiquitin carrier protein B Ubiquitin protein ligase B |
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Endosome,Lysosome |
WB
Western blot analysis of UBE2A/2B using anti-UBE2A/2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBE2A/2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of UBE2A/2B using anti-UBE2A/2B antibody. UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-UBE2A/2B Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HeLa cells using anti-UBE2A/2B antibody. Overlay histogram showing HeLa cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
FC
Flow Cytometry analysis of NIH3T3 cells using anti-UBE2A/2B antibody. Overlay histogram showing NIH3T3 cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
FC
Flow Cytometry analysis of PC-12 cells using anti-UBE2A/2B antibody. Overlay histogram showing PC-12 cells stained with anti-UBE2A/2B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
