WB
SREBP-1 Antibody (2A4) Alexa Fluor® 680:AF680. Direct near-infrared Western blot analysis of SREBP-1 expression in A549 whole cell lysate (A) and HeLa nuclear extract (B). Blocked with UltraCruz® Blocking Reagent: Cruz Marker™ molecular weight standard: detected with Cruz Marker™ MW Tag-Alexa Fluor® 680.WB
SREBP-1 Antibody (2A4). Western blot analysis of SREBP-1 precursor expression in nuclear extracts of HeLa (A), NIH/3T3 (B), and KNRK (C) cells.IHC
SREBP-1 Antibody (2A4). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human adrenal tissue shows cytoplasmic staining in glandular cells.| Product Name | Mouse Monoclonal antibody to SREBP-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 301-407 of SREBP-1a of human origin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HamsterHumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Gene Synonyms | HMD IFAP2 SREBP1 bHLHd1 |
| Gene Full Name | sterol regulatory element binding transcription factor 1 |
| Gene Summary | This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] |
| Molecular Weight(MW) | 125 kDa |
| Cellular Localization | Cytoplasmic vesicle,Endoplasmic reticulum,Golgi apparatus,Membrane,Nucleus |
WB
SREBP-1 Antibody (2A4) Alexa Fluor® 680:AF680. Direct near-infrared Western blot analysis of SREBP-1 expression in A549 whole cell lysate (A) and HeLa nuclear extract (B). Blocked with UltraCruz® Blocking Reagent: Cruz Marker™ molecular weight standard: detected with Cruz Marker™ MW Tag-Alexa Fluor® 680.
WB
SREBP-1 Antibody (2A4). Western blot analysis of SREBP-1 precursor expression in nuclear extracts of HeLa (A), NIH/3T3 (B), and KNRK (C) cells.
IHC
SREBP-1 Antibody (2A4). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human adrenal tissue shows cytoplasmic staining in glandular cells.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
