WB
HT-1080 cells were subjected to SDS PAGE followed by western blot with AGPAT9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.WB
rat kidney tissue were subjected to SDS PAGE followed by western blot with AGPAT9 Antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney using AGPAT9 antibody at dilution of 1:50 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using AGPAT9 Antibody at dilution of 1:200 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using AGPAT9 Antibody at dilution of 1:200 (under 40x lens).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AGPAT9 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).FC(INTRA)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AGPAT9 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | GPAT3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human AGPAT9 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:3000 IHC:1:50-1:400 ICC/IF:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPAT3 |
|---|---|
| Gene Synonyms | MAG1 AGPAT8 AGPAT9 AGPAT10 AGPAT 10 HMFN0839 LPAAT-theta |
| Gene Full Name | glycerol-3-phosphate acyltransferase 3 |
| Gene Summary | This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012] |
| Alternative Names | AGPAT9 1 AGP acyltransferase 9 1 AGPAT 9 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9 |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane |
WB
HT-1080 cells were subjected to SDS PAGE followed by western blot with AGPAT9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB
rat kidney tissue were subjected to SDS PAGE followed by western blot with AGPAT9 Antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney using AGPAT9 antibody at dilution of 1:50 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using AGPAT9 Antibody at dilution of 1:200 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using AGPAT9 Antibody at dilution of 1:200 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AGPAT9 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC(INTRA)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AGPAT9 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:500-1:3000 IHC:1:50-1:400 ICC/IF:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
