WB
Western blot analysis of extracts of Mouse heart cells, using ADCYAP1R1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.WB
Western blot analysis of extracts of U-87MG cells, using ADCYAP1R1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.ICC/IF
Immunofluorescence analysis of SH-SY5Y using ADCYAP1R1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3 using ADCYAP1R1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.| Product Name | ADCYAP1R1 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human ADCYAP1R1(NP_001109.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADCYAP1R1 |
|---|---|
| Gene Synonyms | PAC1 PAC1R PACAPR PACAPRI |
| Gene Full Name | ADCYAP receptor type I |
| Gene Summary | This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010] |
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cell membrane,Membrane |
WB
Western blot analysis of extracts of Mouse heart cells, using ADCYAP1R1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
WB
Western blot analysis of extracts of U-87MG cells, using ADCYAP1R1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
ICC/IF
Immunofluorescence analysis of SH-SY5Y using ADCYAP1R1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3 using ADCYAP1R1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
