WB
Western blot analysis of NOVA1 using anti-NOVA1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOVA1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in frozen section of human placenta tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.ICC/IF
ICC/IF analysis of NOVA1 using anti-NOVA1 antibody and anti-Beta Tubulin antibody. NOVA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro594-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of U87 cells using anti-NOVA1 antibody. Overlay histogram showing U87 cells stained with anti-NOVA1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOVA1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to NOVA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human Nova1 recombinant protein (Position: T413-V506). Human Nova1 shares 100% amino acid (aa) sequence identity with both mouse and rat Nova1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOVA1 |
|---|---|
| Gene Synonyms | Nova-1 |
| Gene Full Name | NOVA alternative splicing regulator 1 |
| Gene Summary | This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | NOVA alternative splicing regulator 1 Nova-1 NOVA1 |
| Molecular Weight(MW) | 52 kDa(Observed band size: 52-75 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of NOVA1 using anti-NOVA1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOVA1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in frozen section of human placenta tissue. The tissue section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of NOVA1 using anti-NOVA1 antibody. NOVA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.
ICC/IF
ICC/IF analysis of NOVA1 using anti-NOVA1 antibody and anti-Beta Tubulin antibody. NOVA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NOVA1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro594-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of U87 cells using anti-NOVA1 antibody. Overlay histogram showing U87 cells stained with anti-NOVA1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOVA1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
