WB
Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-CPNE1 at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;IHC
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB stainingIHC
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB stainingIHC
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Product Name | CPNE1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human CPNE1 : 1-120/537 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:100-1:500 WB:1:500-1:2000 |
|
| Species Reactivity | CowHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CPNE1 |
|---|---|
| Gene Synonyms | CPN1 COPN1 |
| Gene Full Name | copine 1 |
| Gene Summary | Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008] |
| Alternative Names | COPN1 CPN1 1810028N16Rik mKIAA4108 CPNE1_HUMAN CPNE1 Chromobindin 17 Copine I CPNE1_MOUSE copine 1 |
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Nucleus |
WB
Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-CPNE1 at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
IHC
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
IHC
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
IHC
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Application Notes | IHC:1:100-1:500 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
