WB
Western blot: All lanes: ATP6V1E1 antibody at 2µg/ml + 293T whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1/15000 dilution.ICC/IF
Immunofluorescent analysis of Hela cells using ATP6V1E1 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-ATP6V1E1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human V-type proton ATPase subunit E 1 protein (2-224AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ATP6V1E1 |
|---|---|
| Gene Synonyms | P31 Vma4 ATP6E ARCL2C ATP6E2 ATP6V1E |
| Gene Full Name | ATPase H+ transporting V1 subunit E1 |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATP6V1E1 antibody ATP6E antibody ATP6E2 antibody V-type proton ATPase subunit E 1 antibody V-ATPase subunit E 1 antibody V-ATPase 31 kDa subunit antibody p31 antibody Vacuolar proton pump subunit E 1 antibody |
| Molecular Weight(MW) | 27,24,23 kDa(Observed band size: 36 kDa) |
| Cellular Localization | Apical cell membrane. |
WB
Western blot: All lanes: ATP6V1E1 antibody at 2µg/ml + 293T whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1/15000 dilution.
ICC/IF
Immunofluorescent analysis of Hela cells using ATP6V1E1 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
