WB
Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.FC
Flow cytometric analysis of HeLa cells labeling FRS2. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | FRS2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human FRS2 aa 91-240 / 508. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:1000 FC:1:500-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FRS2 |
|---|---|
| Gene Synonyms | SNT SNT1 FRS1A FRS2A SNT-1 FRS2alpha |
| Gene Full Name | fibroblast growth factor receptor substrate 2 |
| Gene Summary | Enables fibroblast growth factor receptor binding activity and neurotrophin TRKA receptor binding activity. Involved in negative regulation of cardiac muscle cell differentiation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in adherens junction. Biomarker of renal cell carcinoma. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | FGFR signaling adaptor SNT antibody FGFR signalling adaptor antibody FGFR substrate 2 antibody FGFR-signaling adaptor SNT antibody Fibroblast growth factor receptor substrate 2 antibody FRS 2 antibody FRS2 antibody FRS2_HUMAN antibody FRS2A antibody FRS2alpha antibody FGFR signaling adaptor SNT antibody FGFR signalling adaptor antibody FGFR substrate 2 antibody FGFR-signaling adaptor SNT antibody Fibroblast growth factor receptor substrate 2 antibody FRS 2 antibody FRS2 antibody FRS2_HUMAN antibody FRS2A antibody FRS2alpha antibody SNT 1 antibody SNT antibody SNT-1 antibody SNT1 antibody Suc 1 Associated Neurotrophic Factor Target antibody Suc1 associated neurotrophic factor target 1 antibody Suc1-associated neurotrophic factor target 1 antibody |
| Molecular Weight(MW) | 57 kDa(Observed band size: 70 kDa ) |
| Cellular Localization | Endomembrane system. |
WB
Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
FC
Flow cytometric analysis of HeLa cells labeling FRS2. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
