WB
Various lysates were subjected to SDS PAGE followed by western blot with MCTS1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MCTS1 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | MCTS1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MCTS1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MCTS1 |
|---|---|
| Gene Synonyms | MCT1 MCT-1 IMD118 |
| Gene Full Name | MCTS1 re-initiation and release factor |
| Gene Summary | Enables RNA cap binding activity; ribosomal small subunit binding activity; and translation factor activity, non-nucleic acid binding. Involved in IRES-dependent viral translational initiation; cytoplasmic translational initiation; and ribosome disassembly. Is active in cytoplasm. Implicated in immunodeficiency 118. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Multiple copies T-cell malignancies MCT-1 MCT1 Malignant T-cell-amplified sequence 1 1F10C7 |
| Molecular Weight(MW) | 19 kDa(Observed band size: 21 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MCTS1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MCTS1 antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:2000-1:10000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
