WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using ASGR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using ASGR1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using ASGR1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-ASGR1 (IP:4ug; Detection:1:2000) with mouse liver tissue lysate 4000 ug.FC
1x10^6 HepG2 cells were surface stained with 0.4 ug ASGR1 Polyclonal antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were not fixed.IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using ASGR1 antibody labeled with FlexAble CoraLite Plus 555 Kit (orange), CoraLite® Plus 488 CD163 antibody (green), CD34 antibody at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | ASGR1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ASGR1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCIPWB |
| WB:1:5000-1:50000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:1000-1:4000 IF-P :1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASGR1 |
|---|---|
| Gene Synonyms | HL-1 ASGPR ASGPR1 CLEC4H1 |
| Gene Full Name | asialoglycoprotein receptor 1 |
| Gene Summary | This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| Alternative Names | asialoglycoprotein receptor 1 HL-1 ASGP R 1 ASGPR ASGPR 1 |
| Molecular Weight(MW) | 33 kDa (Observed band size: 38-42 kDa) |
| Cellular Localization | Cell membrane,Membrane,Secreted |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using ASGR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using ASGR1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using ASGR1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-ASGR1 (IP:4ug; Detection:1:2000) with mouse liver tissue lysate 4000 ug.
FC
1x10^6 HepG2 cells were surface stained with 0.4 ug ASGR1 Polyclonal antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were not fixed.
IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using ASGR1 antibody labeled with FlexAble CoraLite Plus 555 Kit (orange), CoraLite® Plus 488 CD163 antibody (green), CD34 antibody at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using ASGR1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:5000-1:50000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:1000-1:4000 IF-P :1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
