WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
L02 cells were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
WB result of ASGR1 antibody (1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using ASGR1 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using ASGR1 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ASGR1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ASGR1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC :1:500-1:2000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ASGR1 |
|---|---|
| Gene Synonyms | HL-1 ASGPR ASGPR1 CLEC4H1 |
| Gene Full Name | asialoglycoprotein receptor 1 |
| Gene Summary | This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| Alternative Names | asialoglycoprotein receptor 1 HL-1 Asgpr1 ASGP-R 1 ASGPR 1 |
| Molecular Weight(MW) | 33 kDa (Observed band size: 42-46 kDa) |
| Cellular Localization | Cell membrane,Membrane,Secreted |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
L02 cells were subjected to SDS PAGE followed by western blot with ASGR1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
WB result of ASGR1 antibody (1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using ASGR1 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using ASGR1 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:2000-1:10000 IHC :1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
