WB
Western blot analysis of various lysates using ALOX15 Rabbit pAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.| Product Name | ALOX15 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:5000 IHC:1:5000-1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALOX15 |
|---|---|
| Gene Synonyms | LOG15 12-LOX 15-LOX 15-LOX-1 |
| Gene Full Name | arachidonate 15-lipoxygenase |
| Gene Summary | This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | LOG15 12-LOX 15-LOX 15-LOX-1 ALOX15 |
| Molecular Weight(MW) | 75 kDa/70 kDa(Observed band size: 75 kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, Lipid droplet, Peripheral membrane protein, cytosol. |
WB
Western blot analysis of various lysates using ALOX15 Rabbit pAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using ALOX15 Rabbit pAb at a dilution of 1:15000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.| Application Notes | WB:1:2000-1:5000 IHC:1:5000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. |
