WB
Western blot analysis of Histone H3.3 (mutated G34R) on different lysates with Rabbit anti-Histone H3.3 (mutated G34R) antibody at 1/20,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-Histone H3.3 (mutated G34R) antibody, 1/20,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureIHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: 293T transfected with Histone H3.3 G34R expression vector. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue:Wild-type 293T（Negative control）. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: 293T transfected with Histone H3.3 G34V expression vector（Negative control）. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Product Name | Histone H3.3 (mutated G34R) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:20000 IHC:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H3-3A |
|---|---|
| Gene Synonyms | H3F3 H3.3A H3F3A BRYLIB1 |
| Gene Full Name | H3.3 histone A |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 20 kDa) |
| Cellular Localization | Nucleus, Chromosome. |
| SwissProt ID | P84243 |
|---|
WB
Western blot analysis of Histone H3.3 (mutated G34R) on different lysates with Rabbit anti-Histone H3.3 (mutated G34R) antibody at 1/20,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-Histone H3.3 (mutated G34R) antibody, 1/20,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: 293T transfected with Histone H3.3 G34R expression vector. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue:Wild-type 293T（Negative control）. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: 293T transfected with Histone H3.3 G34V expression vector（Negative control）. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/5,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Application Notes | WB:1:20000 IHC:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
