WB
Western blot analysis of various lysates using Histone H2B (testis specific) Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Mouse brain,Rat brain. Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using Histone H2B (testis specific) Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Mouse testis using Histone H2B (testis specific) Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.IF-P
Confocal imaging of paraffin-embedded Human testis using Histone H2B (testis specific) Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.IP
Immunoprecipitation of Histone H2B (testis specific) from 600 µg extracts of Rat testis tissue was performed using 2 µg of Histone H2B (testis specific) Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Histone H2B (testis specific) Rabbit mAb at a dilution of 1:1000.CHIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from mouse testis, using Histone H2B(testis specific) Rabbit mAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.| Product Name | Histone H2B (testis specific) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPIF-PIHCIPWB |
| WB:1:2000-1:6000 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells IF-P:1:200-1:800 IHC:1:200-1:2000 ChIP:5μg antibody for 5μg-15μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2BC1 |
|---|---|
| Gene Synonyms | STBP TH2B H2BFU TSH2B hTSH2B TSH2B.1 HIST1H2BA bA317E16.3 |
| Gene Full Name | H2B clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 14 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | Q96A08 |
|---|
WB
Western blot analysis of various lysates using Histone H2B (testis specific) Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Mouse brain,Rat brain. Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using Histone H2B (testis specific) Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Mouse testis using Histone H2B (testis specific) Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
IF-P
Confocal imaging of paraffin-embedded Human testis using Histone H2B (testis specific) Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
IP
Immunoprecipitation of Histone H2B (testis specific) from 600 µg extracts of Rat testis tissue was performed using 2 µg of Histone H2B (testis specific) Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Histone H2B (testis specific) Rabbit mAb at a dilution of 1:1000.
CHIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from mouse testis, using Histone H2B(testis specific) Rabbit mAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.| Application Notes | WB:1:2000-1:6000 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells IF-P:1:200-1:800 IHC:1:200-1:2000 ChIP:5μg antibody for 5μg-15μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
