WB
Western blot analysis of ACTN4 using anti-ACTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of ACTN4 using anti-ACTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.IHC
IHC analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Hepa1-6 cells using anti-ACTN4 antibody. Overlay histogram showing Hepa1-6 cells stained with anti-ACTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.FC
Flow Cytometry analysis of C6 cells using anti-ACTN4 antibody. Overlay histogram showing C6 cells stained with anti-ACTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ACTN4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 FC:1:50-1:200 ICC/IF:1:50-1:400 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN4 |
|---|---|
| Gene Synonyms | FSGS FSGS1 ACTININ-4 |
| Gene Full Name | actinin alpha 4 |
| Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | ACTININ 4 actinin alpha 4 ACTN4 Alpha actinin 4 F actin cross linking protein FSGS FSGS1 Non muscle alpha actinin 4 |
| Molecular Weight(MW) | 105 kDa |
| Cellular Localization | Cell junction,Cytoplasm,Cytoskeleton,Nucleus |
WB
Western blot analysis of ACTN4 using anti-ACTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of ACTN4 using anti-ACTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC)with DAB as the chromogen.
IHC
IHC analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ACTN4 using anti-ACTN4 antibody. ACTN4 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ACTN4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Hepa1-6 cells using anti-ACTN4 antibody. Overlay histogram showing Hepa1-6 cells stained with anti-ACTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
FC
Flow Cytometry analysis of C6 cells using anti-ACTN4 antibody. Overlay histogram showing C6 cells stained with anti-ACTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 ICC/IF:1:50-1:400 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
