WB
Western Blot: Positive WB detected in: HeLa whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A-431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Jurkat whole cell lysate(30µg). All lanes: ACTN4 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Exposure time: 1min.Immunofluorescence staining of Hela cell with ACTN4 Recombinant Monoclonal Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with ACTN4 Recombinant Monoclonal Antibody (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
| Product Name | ACTN4 Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human ACTN4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN4 |
|---|---|
| Gene Synonyms | FSGS FSGS1 ACTININ-4 |
| Gene Full Name | actinin alpha 4 |
| Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | actinin 4 antibody Actinin alpha 4 antibody actinin4 antibody ACTN 4 antibody ACTN4 antibody ACTN4_HUMAN antibody Alpha-actinin-4 antibody DKFZp686K23158 antibody F actin cross linking protein antibody F-actin cross-linking protein antibody Focal segmental glomerulosclerosis 1 antibody FSGS 1 antibody FSGS antibody FSGS1 antibody Non muscle alpha actinin 4 antibody Non-muscle alpha-actinin 4 antibody |
| Molecular Weight(MW) | 105 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Cell junction. Cytoplasm, cytoskeleton, stress fiber. Cytoplasm, perinuclear region. |
WB
Western Blot: Positive WB detected in: HeLa whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A-431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Jurkat whole cell lysate(30µg). All lanes: ACTN4 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Exposure time: 1min.
Immunofluorescence staining of Hela cell with ACTN4 Recombinant Monoclonal Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with ACTN4 Recombinant Monoclonal Antibody (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
