WB
Western blot analysis of extracts of various cell lines, using PTK7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.IHC
Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Product Name | PTK7 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polyclonal Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500 - 1:2000 IHC:1:50 - 1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK7 |
|---|---|
| Gene Synonyms | CCK4 CCK-4 |
| Gene Full Name | protein tyrosine kinase 7 (inactive) |
| Gene Summary | This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| Molecular Weight(MW) | 160 KDa |
| Cellular Localization | Cell junction,Cell membrane,Membrane |
WB
Western blot analysis of extracts of various cell lines, using PTK7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
IHC
Immunohistochemistry of paraffin-embedded mouse brain using PTK7 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry of paraffin-embedded human prostate using PTK7 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Application Notes | WB:1:500 - 1:2000 IHC:1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
