WB
Relative expression (RE) Western blot analysis of CCK4 on different lysates with Mouse anti-CCK4 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CCK4 with Mouse anti-CCK4 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Product Name | CCK4 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CCK4 aa 31-704 / 1,070. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:500 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTK7 |
|---|---|
| Gene Synonyms | CCK4 CCK-4 |
| Gene Full Name | protein tyrosine kinase 7 (inactive) |
| Gene Summary | This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| Alternative Names | 8430404F20Rik antibody CCK 4 antibody CCK-4 antibody Colon carcinoma kinase 4 antibody Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody Inactive tyrosine-protein kinase 7 antibody mPTK7/CCK4 antibody OTTHUMP00000217016 antibody Protein tyrosine kinase PTK 7 antibody Protein-tyrosine kinase 7 antibody 8430404F20Rik antibody CCK 4 antibody CCK-4 antibody Colon carcinoma kinase 4 antibody Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody Inactive tyrosine-protein kinase 7 antibody mPTK7/CCK4 antibody OTTHUMP00000217016 antibody Protein tyrosine kinase PTK 7 antibody Protein-tyrosine kinase 7 antibody Pseudo tyrosine kinase receptor 7 antibody PTK 7 antibody PTK7 antibody PTK7 protein tyrosine kinase 7 antibody PTK7 protein tyrosine kinase antibody PTK7_HUMAN antibody Serum response factor antibody Srf antibody Tyrosine-protein kinase-like 7 antibody |
| Molecular Weight(MW) | 118 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Membrane, Cell junction. |
WB
Relative expression (RE) Western blot analysis of CCK4 on different lysates with Mouse anti-CCK4 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CCK4 with Mouse anti-CCK4 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
