WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-CD86 antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureIF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Antibody A: 1/200 (CD86, rabbit, Red), Antibody B: 1/200 (CD68, rat, Green), overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (594), Goat anti-Rat IgG (488), 1.5 hours at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Liver. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Skeletal muscle. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature. Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern.| Product Name | CD86 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse CD86 aa 1-265. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IF-P:1:200 IHC:1:200-1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Cd86 |
|---|---|
| Gene Synonyms | B7 B70 MB7 B7-2 B7.2 CLS1 Ly58 ETC-1 Ly-58 MB7-2 Cd28l2 TS/A-2 |
| Gene Full Name | CD86 antigen |
| Gene Summary | Predicted to enable receptor ligand activity. Involved in several processes, including positive regulation of immunoglobulin production; positive regulation of non-canonical NF-kappaB signal transduction; and toll-like receptor 3 signaling pathway. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of T cell proliferation; and toll-like receptor signaling pathway. Located in external side of plasma membrane and intracellular membrane-bounded organelle. Is expressed in central nervous system; mandible; and retina. Used to study Guillain-Barre syndrome. Human ortholog(s) of this gene implicated in several diseases, including IgA vasculitis; autoimmune disease (multiple); chronic lymphocytic leukemia; chronic obstructive pulmonary disease; and systemic scleroderma. Orthologous to human CD86 (CD86 molecule). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antibody Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antibody B72 antigen antibody BU63 antibody CD28 antigen ligand 2 2 antibody CD28 antigen ligand 2 antibody Cd28l2 antibody CD28LG2 antibody CD86 antibody CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) 1 2 antibody CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) antibody CD86 antigen antibody CD86 molecule antibody CD86_HUMAN antibody CLS1 antibody CTLA-4 counter-receptor B7.2 2 antibody CTLA-4 counter-receptor B7.2 2 3 antibody CTLA-4 counter-receptor B7.2 antibody Early T-cell costimulatory molecule 1 antibody ETC-1 antibody FUN 1 antibody FUN-1 antibody FUN1 antibody LAB72 antibody Ly-58 antibody Ly58 antibody MB7 antibody MB7-2 antibody Membrane glycoprotein antibody MGC34413 antibody T lymphocyte activation antigen CD86 precursor antibody T-lymphocyte activation antigen CD86 antibody TS/A-2 antibody |
| Molecular Weight(MW) | 35 kDa(Observed band size: 60-85 kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Rabbit anti-CD86 antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Antibody A: 1/200 (CD86, rabbit, Red), Antibody B: 1/200 (CD68, rat, Green), overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (594), Goat anti-Rat IgG (488), 1.5 hours at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Liver. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Skeletal muscle. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature. Negative expression of CD86 protein in skeletal muscle is consistent with the predicted expression pattern.| Application Notes | WB:1:5000 IF-P:1:200 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
