WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using FYN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using FYN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using FYN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using FYN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | FYN Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FYN protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:8000 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FYN |
|---|---|
| Gene Synonyms | SLK SYN p59-FYN |
| Gene Full Name | FYN proto-oncogene, Src family tyrosine kinase |
| Gene Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] |
| Alternative Names | Proto-oncogene c-Fyn Proto oncogene Syn Proto oncogene c Fyn p59-Fyn p59 Fyn |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using FYN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using FYN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using FYN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using FYN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:8000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
