WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using FYN antibody at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using FYN antibody at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using FYN antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug FYN Monoclonal antibody and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | FYN Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FYN protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:30000 IHC :1:2000-1:5000 ICC/IF:1:400-1:1600 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | FYN |
|---|---|
| Gene Synonyms | SLK SYN p59-FYN |
| Gene Full Name | FYN proto-oncogene, Src family tyrosine kinase |
| Gene Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1G4B1 EC:2.7.10.2 p59 Fyn p59-Fyn Proto oncogene c Fyn |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with FYN antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using FYN antibody at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using FYN antibody at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using FYN antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using FYN antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug FYN Monoclonal antibody and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:5000-1:30000 IHC :1:2000-1:5000 ICC/IF:1:400-1:1600 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
