WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen IV antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.WB
Western blot analysis of lysates from HeLa cells, using Collagen IV Antibody. The lane on the right is blocked with the synthesized peptide.IHC
Human appendix was stained with Anti-Collagen IV rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Collagen IV antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Product Name | Collagen IV Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IHC:1:200-1000 WB:1:1000-5000 ICC/IF:1:200-1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COL4A1 |
|---|---|
| Gene Synonyms | BSVD BSVD1 RATOR PADMAL COL4A1s |
| Gene Full Name | collagen type IV alpha 1 chain |
| Gene Summary | This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | Arresten BSVD CO4A1_HUMAN COL4A1 COL4A1 NC1 domain COL4A2 COL4A3 COL4A4 COL4A5 collagen alpha-1 IV chain Collagen IV Alpha 1 Polypeptide Collagen IV Alpha 2 Polypeptide Collagen Of Basement Membrane Alpha 1 Chain Collagen Of Basement Membrane Alpha 2 Chain Collagen Type IV Alpha 1 collagen type IV alpha 1 chain Collagen Type IV Alpha 2 Collagen Type IV Alpha 3 Collagen Type IV Alpha 4 Collagen Type IV Alpha 5 RatOR |
| Molecular Weight(MW) | 160 kDa(Observed band size: 200 kDa) |
| Cellular Localization | Cytoplasmic |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen IV antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
WB
Western blot analysis of lysates from HeLa cells, using Collagen IV Antibody. The lane on the right is blocked with the synthesized peptide.
IHC
Human appendix was stained with Anti-Collagen IV rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Collagen IV antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Application Notes | IHC:1:200-1000 WB:1:1000-5000 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
