WB
Western blot analysis of YTHDC1 using anti-YTHDC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of YTHDC1 using anti-YTHDC1 antibody. YTHDC1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-YTHDC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of YTHDC1 using anti-YTHDC1 antibody and anti-Alpha Tubulin antibody. YTHDC1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-YTHDC1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488 conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.IP
IP analysis of YTHDC1 using anti-YTHDC1 antibody in RT4 whole cell lysate. Western blot analysis of YTHDC1 using anti- YTHDC1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- YTHDC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to YTHDC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human YTHDC1 recombinant protein (Position: S326-K651). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YTHDC1 |
|---|---|
| Gene Synonyms | YT521 YT521-B |
| Gene Full Name | YTH N6-methyladenosine RNA binding protein C1 |
| Gene Summary | Enables N6-methyladenosine-containing RNA reader activity and RNA binding activity. Involved in gene expression and regulation of gene expression. Located in nuclear speck and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | KIAA1966 Putative splicing factor YT521 YT521 YT521 B YTH domain containing 1 YTHDC1 |
| Molecular Weight(MW) | 85 kDa(Observed band size:110 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of YTHDC1 using anti-YTHDC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of YTHDC1 using anti-YTHDC1 antibody. YTHDC1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-YTHDC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of YTHDC1 using anti-YTHDC1 antibody and anti-Alpha Tubulin antibody. YTHDC1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-YTHDC1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488 conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
IP
IP analysis of YTHDC1 using anti-YTHDC1 antibody in RT4 whole cell lysate. Western blot analysis of YTHDC1 using anti- YTHDC1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- YTHDC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
