WB
human brain tissue were subjected to SDS PAGE followed by western blot with ATP6V1D antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with ATP6V1D antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using ATP6V1D antibody at dilution of 1:100 (under 40x lens).IP
IP result of anti-ATP6V1D (IP: 4ug; Detection: 1:500) with mouse lung tissue lysate 4000ug.| Product Name | ATP6V1D Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ATP6V1D protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2400 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP6V1D |
|---|---|
| Gene Synonyms | VATD VMA8 ATP6M |
| Gene Full Name | ATPase H+ transporting V1 subunit D |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATP6M ATP6V1D V ATPase subunit D V type proton ATPase subunit D Vacuolar proton pump subunit D VATD VMA8 |
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cell projection,Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Lysosome,Membrane |
WB
human brain tissue were subjected to SDS PAGE followed by western blot with ATP6V1D antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ATP6V1D antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using ATP6V1D antibody at dilution of 1:100 (under 40x lens).
IP
IP result of anti-ATP6V1D (IP: 4ug; Detection: 1:500) with mouse lung tissue lysate 4000ug.| Application Notes | WB:1:500-1:2400 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
