WB
Western blot analysis of PUS7 using anti-PUS7 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PUS7 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogenIHC
HC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of rat bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).ICC/IF
ICC/IF analysis of PUS7 using anti-PUS7 antibody and anti-Beta Tubulin antibody. PUS7 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of MCF-7 cells using anti-PUS7 antibody . Overlay histogram showing MCF-7 cells stained with Rabbit polyclonal antibody to PUS7 (Blue line). To facilitate intrPUS7llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUS7 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PUS7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human PUS7 recombinant protein (Position: D294-H541). Human PUS7 shares 92.7% amino acid (aa) sequence identity with mouse PUS7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUS7 |
|---|---|
| Gene Synonyms | IDDABS |
| Gene Full Name | pseudouridine synthase 7 |
| Gene Summary | Enables enzyme binding activity and tRNA pseudouridine(13) synthase activity. Involved in several processes, including pseudouridine synthesis; regulation of hematopoietic stem cell differentiation; and regulation of mesoderm development. Is active in nucleus. Implicated in intellectual developmental disorder with abnormal behavior, microcephaly, and short stature. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | PUS7 KIAA1897 Pseudouridylate synthase 7 homolog |
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Nucleus |
WB
Western blot analysis of PUS7 using anti-PUS7 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PUS7 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen
IHC
HC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of rat bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of PUS7 using anti-PUS7 antibody. PUS7 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
ICC/IF
ICC/IF analysis of PUS7 using anti-PUS7 antibody and anti-Beta Tubulin antibody. PUS7 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PUS7 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of MCF-7 cells using anti-PUS7 antibody . Overlay histogram showing MCF-7 cells stained with Rabbit polyclonal antibody to PUS7 (Blue line). To facilitate intrPUS7llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUS7 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
