WB
Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | DGAT1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human DGAT1 aa 1-150 / 488. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DGAT1 |
|---|---|
| Gene Synonyms | ARAT DGAT ARGP1 DIAR7 |
| Gene Full Name | diacylglycerol O-acyltransferase 1 |
| Gene Summary | This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013] |
| Alternative Names | ACAT related gene product 1 antibody ACAT-related gene product 1 antibody Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody Acyl-CoA retinol O-fatty-acyltransferase antibody Acyl-CoA:diacylglycerol acyltransferase antibody ARAT antibody ARGP1 antibody C75990 antibody D15Ertd23e antibody Dgat antibody ACAT related gene product 1 antibody ACAT-related gene product 1 antibody Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody Acyl-CoA retinol O-fatty-acyltransferase antibody Acyl-CoA:diacylglycerol acyltransferase antibody ARAT antibody ARGP1 antibody C75990 antibody D15Ertd23e antibody Dgat antibody DGAT1 antibody DGAT1_HUMAN antibody Diacylglycerol O acyltransferase 1 antibody Diacylglycerol O-acyltransferase 1 antibody DIAR7 antibody Diglyceride acyltransferase antibody EC 2.3.1.20 antibody hCG_24006 antibody MGC139064 antibody Retinol O fatty acyltransferase antibody |
| Molecular Weight(MW) | 55 kDa(Observed band size: 48 kDa) |
| Cellular Localization | Endoplasmic reticulum,Membrane |
WB
Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
