WB
Western blot analysis of anti-MOGAT2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MOGAT2 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MOGAT2 using anti-MOGAT2 antibody. MOGAT2 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of MOGAT2 using anti-MOGAT2 antibody. MOGAT2 was detected in an immunocytochemical section of HepG2 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-MOGAT2 antibody. Overlay histogram showing HepG2 cells stained with Anti-MOGAT2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOGAT2 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to MOGAT2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human MOGAT2 recombinant protein (Position: M1-C334). Human MOGAT2 shares 81.1% amino acid (aa) sequence identity with mouse MOGAT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MOSPD2 |
|---|---|
| Gene Full Name | motile sperm domain containing 2 |
| Gene Summary | Enables FFAT motif binding activity. Involved in lipid droplet formation; positive regulation of leukocyte chemotaxis; and protein homooligomerization. Located in endoplasmic reticulum membrane; endoplasmic reticulum-endosome membrane contact site; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DC5 DGAT2L5 hDC5 hMGAT2 MGAT2 MOGAT2 |
| Molecular Weight(MW) | 38 kDa(Observed band size:43 kDa) |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Membrane. |
WB
Western blot analysis of anti-MOGAT2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MOGAT2 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MOGAT2 using anti-MOGAT2 antibody. MOGAT2 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of MOGAT2 using anti-MOGAT2 antibody. MOGAT2 was detected in an immunocytochemical section of HepG2 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-MOGAT2 antibody. Overlay histogram showing HepG2 cells stained with Anti-MOGAT2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOGAT2 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
