IHC
IHC staining of human brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded human brain tissue slide using MAP2 Monoclonal antibody at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of mouse brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using MAP2 Monoclonal antibodyat dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of rat brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded rat brain tissue slide usingMAP2 Monoclonal antibody at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
IF Staining of mouse brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IF-P
IF Staining of rat brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IF-F
IF Staining of mouse brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L), 594 GFAP antibody (red), TDP-43 antibody (10782-2-AP, Magenta).IF-F
IF Staining of rat brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L), GFAP antibody (red).FC
FC experiment of Neuro-2a using MAP2 Monoclonal antibody 1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | MAP2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MAP2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCIF-FIF-PIHC |
| IHC:1:1000-1:4000 IF-P:1:200-1:800 IF-F:1:1000-1:4000 FC:0.40 ug per 10^6 cells in a 100 µl suspension. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP2 |
|---|---|
| Gene Synonyms | MAP-2 MAP2A MAP2B MAP2C Summary This gene encodes a protein that belongs t |
| Gene Full Name | microtubule associated protein 2 |
| Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010] |
| Alternative Names | 1C3E6 MAP 2 MAP-2 MAP2A MAP2B |
| Molecular Weight(MW) | 200 kDa |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Microtubule. |
IHC
IHC staining of human brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded human brain tissue slide using MAP2 Monoclonal antibody at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of mouse brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using MAP2 Monoclonal antibodyat dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of rat brain using MAP2 Monoclonal antibody Immunohistochemical analysis of paraffin-embedded rat brain tissue slide usingMAP2 Monoclonal antibody at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
IF Staining of mouse brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF-P
IF Staining of rat brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF-F
IF Staining of mouse brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L), 594 GFAP antibody (red), TDP-43 antibody (10782-2-AP, Magenta).
IF-F
IF Staining of rat brain using MAP2 Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L), GFAP antibody (red).
FC
FC experiment of Neuro-2a using MAP2 Monoclonal antibody 1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | IHC:1:1000-1:4000 IF-P:1:200-1:800 IF-F:1:1000-1:4000 FC:0.40 ug per 10^6 cells in a 100 µl suspension. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
