WB
Western blot analysis of anti- SNRNP27 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SNRNP27 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SNRNP27 using anti-SNRNP27 antibody and anti-Beta Tubulin antibody. SNRNP27 was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of human colon cancer tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.| Product Name | Rabbit polyclonal antibody to SNRNP27 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SNRNP27 recombinant protein (Position: E100-A155). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNRNP27 |
|---|---|
| Gene Synonyms | 27K RY1 |
| Gene Full Name | small nuclear ribonucleoprotein U4/U6.U5 subunit 27 |
| Gene Summary | This gene encodes a serine/arginine-rich (SR) protein. SR proteins play important roles in pre-mRNA splicing by facilitating the recognition and selection of splice sites. The encoded protein associates with the 25S U4/U6.U5 tri-snRNP, a major component of the U2-type spiceosome. The expression of this gene may be altered in cells infected with the human T-cell lymphotropic virus type 1 (HTLV-1) retrovirus. A pseudogene of this gene is located on the long arm of chromosome 5. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011] |
| Alternative Names | U4/U6.U5-27K SNRNP27 |
| Molecular Weight(MW) | 19 kDa(Observed band size:23 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of anti- SNRNP27 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SNRNP27 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SNRNP27 using anti-SNRNP27 antibody and anti-Beta Tubulin antibody. SNRNP27 was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-SNRNP27 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of human colon cancer tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.
IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.
IF-P
IF analysis of SNRNP27 using anti-SNRNP27 antibody. SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
