WB
WB analysis of A431 using DSG3 Recombinant monoclonal antibody A431 cells were subjected to SDS PAGE followed by western blot with DSG3 Recombinant monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. Low expression: SK-MEL-28 cells.WB
WB analysis of HaCaT using DSG3 Recombinant monoclonal antibody HaCaT cells were subjected to SDS PAGE followed by western blot with DSG3 Recombinant monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human tonsillitis using DSG3 Recombinant monoclonal antibody Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using DSG3 Recombinant monoclonal antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of HaCaT using DSG3 Recombinant monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | DSG3 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human DSG3 protein (rFc Tag) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DSG3 |
|---|---|
| Gene Synonyms | PVA ABOLM CDHF6 |
| Gene Full Name | desmoglein 3 |
| Gene Summary | This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris. [provided by RefSeq, Jan 2016] |
| Alternative Names | Cadherin family member 6 CDHF6 Desmoglein 3 Desmoglein-3 DSG 3 |
| Molecular Weight(MW) | 108 kDa(Observed band size:130 kDa ) |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Membrane,Tight junction. |
WB
WB analysis of A431 using DSG3 Recombinant monoclonal antibody A431 cells were subjected to SDS PAGE followed by western blot with DSG3 Recombinant monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. Low expression: SK-MEL-28 cells.
WB
WB analysis of HaCaT using DSG3 Recombinant monoclonal antibody HaCaT cells were subjected to SDS PAGE followed by western blot with DSG3 Recombinant monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human tonsillitis using DSG3 Recombinant monoclonal antibody Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using DSG3 Recombinant monoclonal antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of HaCaT using DSG3 Recombinant monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using DSG3 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:250-1:1000 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
