WB
Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Skin. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: HaCaT (Human immortalized keratinocyte) / A549 (Human lung adenocarcinoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.FC
Application: Flow Cytometry. Species: Human. Sample: A549 (Human lung adenocarcinoma cell) (Left) / HaCaT (Human immortalized keratinocyte) (Right). Blocking: 1% BSA + 10% normal goat serum , 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody(1/2,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature. Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.| Product Name | Desmoglein 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Desmoglein 3 aa 301-615. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000 IHC:1:200-1:1000 ICC/IF:1:500 FC:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DSG3 |
|---|---|
| Gene Synonyms | PVA ABOLM CDHF6 |
| Gene Full Name | desmoglein 3 |
| Gene Summary | This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris. [provided by RefSeq, Jan 2016] |
| Alternative Names | 130 kD pemphigus vulgaris antigen antibody 130 kDa pemphigus vulgaris antigen antibody Balding antibody Cadherin family member 6 antibody CDHF 6 antibody CDHF6 antibody Desmoglein 3 antibody Desmoglein 3 precursor antibody Desmoglein-3 antibody Desmoglein3 antibody DKFZp686P23184 antibody DSG 3 antibody DSG3 antibody DSG3_HUMAN antibody Pemphigus vulgaris antigen antibody PVA antibody |
| Molecular Weight(MW) | 108 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Cell membrane, Cell junction, desmosome, Cytoplasm, tight junction. |
WB
Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Skin. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: HaCaT (Human immortalized keratinocyte) / A549 (Human lung adenocarcinoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.
FC
Application: Flow Cytometry. Species: Human. Sample: A549 (Human lung adenocarcinoma cell) (Left) / HaCaT (Human immortalized keratinocyte) (Right). Blocking: 1% BSA + 10% normal goat serum , 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: Desmoglein 3 Recombinant Rabbit Monoclonal Antibody(1/2,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature. Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.| Application Notes | WB:1:5000 IHC:1:200-1:1000 ICC/IF:1:500 FC:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
