WB
Western blot analysis of HYCC1 using anti-HYCC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HYCC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HYCC1 using anti-HYCC1 antibody. HYCC1 was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-HYCC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of HYCC1 using anti-HYCC1 antibody and anti-Alpha Tubulin antibody. HYCC1 was detected in an immunocytochemical section of SIHA cells. The section was incubated with rabbit anti-HYCC1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.| Product Name | Rabbit polyclonal antibody to HYCC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human HYCC1 recombinant protein (Position: N110-H464). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HYCC1 |
|---|---|
| Gene Synonyms | HCC HLD5 FAM126A DRCTNNB1A |
| Gene Full Name | hyccin PI4KA lipid kinase complex subunit 1 |
| Gene Summary | The protein encoded by this gene may play a part in the beta-catenin/Lef signaling pathway. Expression of this gene is down-regulated by beta-catenin. Defects in this gene are a cause of hypomyelination with congenital cataract (HCC). [provided by RefSeq, Oct 2008] |
| Alternative Names | FAM126A DRCTNNB1A Hyccin Down-regulated by CTNNB1 protein A Protein FAM126A |
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane. |
WB
Western blot analysis of HYCC1 using anti-HYCC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HYCC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HYCC1 using anti-HYCC1 antibody. HYCC1 was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-HYCC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of HYCC1 using anti-HYCC1 antibody and anti-Alpha Tubulin antibody. HYCC1 was detected in an immunocytochemical section of SIHA cells. The section was incubated with rabbit anti-HYCC1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
