WB
WB analysis using GALNT1 Polyclonal antibody Various lysates were subjected to SDS PAGE followed by western blot with GALNT1 Polyclonal antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
IHC staining of mouse kidney using GALNT1 Polyclonal antibody Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using GALNT1 Polyclonal antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of rat lung using GALNT1 Polyclonal antibody Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using GALNT1 Polyclonal antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | GALNT1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human GALNT1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GALNT1 |
|---|---|
| Gene Synonyms | GALNAC-T1 |
| Gene Full Name | polypeptide N-acetylgalactosaminyltransferase 1 |
| Gene Summary | This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008] |
| Alternative Names | EC:2.4.1.41 GALNAC T1 GalNAc-T1 Polypeptide N-acetylgalactosaminyltransferase 1 Polypeptide N-acetylgalactosaminyltransferase 1 soluble form |
| Molecular Weight(MW) | 559 aa, 64 kDa |
| Cellular Localization | Golgi apparatus,Membrane,Secreted. |
WB
WB analysis using GALNT1 Polyclonal antibody Various lysates were subjected to SDS PAGE followed by western blot with GALNT1 Polyclonal antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
IHC staining of mouse kidney using GALNT1 Polyclonal antibody Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using GALNT1 Polyclonal antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of rat lung using GALNT1 Polyclonal antibody Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using GALNT1 Polyclonal antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:1000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
