WB
Relative expression (RE) Western blot analysis of HCN4 on different lysates with Rabbit anti-HCN4 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Product Name | HCN4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse HCN4 aa 1-263. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HCN4 |
|---|---|
| Gene Synonyms | SSS2 EIG18 BRGDA8 |
| Gene Full Name | hyperpolarization activated cyclic nucleotide gated potassium channel 4 |
| Gene Summary | This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15. [provided by RefSeq, Oct 2008] |
| Alternative Names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Brain cyclic nucleotide-gated channel 3 (BCNG-3) Hcn4 Bcng3 |
| Molecular Weight(MW) | 129 kDa(Observed band size:130-160 kDa) |
| Cellular Localization | Cell membrane. |
WB
Relative expression (RE) Western blot analysis of HCN4 on different lysates with Rabbit anti-HCN4 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
