WB
Western Blot Positive WB detected in: recombinant protein All lanes:Outer capsid glycoprotein VP7 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.| Product Name | Rabbit anti-VP7 (Outer capsid glycoprotein) Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Rotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1]) (RV-A) Outer capsid glycoprotein VP7 protein (34-309aa) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:5000 |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | Outer capsid glycoprotein VP7 (Fragment) |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Function | Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7. |
| Cellular Localization | Virion. Host endoplasmic reticulum lumen. |
WB
Western Blot Positive WB detected in: recombinant protein All lanes:Outer capsid glycoprotein VP7 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.| Application Notes | WB:1:500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
