WB
Sun, Xiao‐Dong, et al. "Regulation of the firing activity by PKA‐PKC‐Src family kinases in cultured neurons of hypothalamic arcuate nucleus." Journal of neuroscience research 98.2 (2020): 384-403.WB
Western blot analysis of lysates from 1) 22RV1, 2) Hela , 3) COLO205 cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PKA alpha/beta CAT Antibody. The picture on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of Hela cell. 1,PKAα/β/γ cat Polyclonal Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Product Name | PKA α/β/γ Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:200-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKACA |
|---|---|
| Gene Synonyms | CAFD1 PKACA PPNAD4 |
| Gene Full Name | protein kinase cAMP-activated catalytic subunit alpha |
| Gene Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] |
| Alternative Names | PRKACA PKACA cAMP-dependent protein kinase catalytic subunit alpha PKA C-alpha PRKACB cAMP-dependent protein kinase catalytic subunit beta PKA C-beta PRKACG cAMP-dependent protein kinase catalytic subunit gamma PKA C-gamma |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane ; Lipid-anchor. |
WB
Sun, Xiao‐Dong, et al. "Regulation of the firing activity by PKA‐PKC‐Src family kinases in cultured neurons of hypothalamic arcuate nucleus." Journal of neuroscience research 98.2 (2020): 384-403.
WB
Western blot analysis of lysates from 1) 22RV1, 2) Hela , 3) COLO205 cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.
IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PKA alpha/beta CAT Antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,PKAα/β/γ cat Polyclonal Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:200-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
